Isolation of Babesia divergens from carrier cattle blood using in vitro culture

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Isolation of Babesia divergens from carrier cattle blood using in vitro culture.

Babesia divergens, the main causative agent of bovine babesiosis in Western Europe, was isolated from naturally infected cattle. Ninety-six blood samples were examined by means of an in vitro culture technique in sheep erythrocytes: 19 of them were collected from animals in the acute phase of the disease with visible parasitemia on blood smears, while the 77 remaining animals showed no microsco...

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Isolation of Bovine Herpesvirus-1 (BoHV-1) From Latently Infected/Carrier Cattle in Ahvaz

Bovine herpesvirus 1 (BoHV-1) is one of the most important infectious viruses of cattle which is considered as the causative agent of several clinical conditions in this species. Hence, we tried to isolate a local strain of the virus from carrier cattle in Ahvaz based on a strategy for reactivation of the latent infection in hosts having antibodies against the virus. Thus, 20 cattle were examin...

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Novel diamidines with activity against Babesia divergens in vitro and Babesia microti in vivo.

Dicationic diamidines, such as diminazene and pentamidine, are well-studied chemotherapeutic agents with significant activity against parasitic diseases. The in vitro activities of novel diamidine compounds against the Babesia divergens strains 1903B and 4201 were investigated. The most potent compound, a diphenyl furan, had a 50% inhibitory concentration (IC(50)) of 1.5 ng/ml. In a murine mode...

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The invasion process of bovine erythrocyte by Babesia divergens: knowledge from an in vitro assay

Babesia divergens is a tick-transmitted apicomplexan parasite for which asexual multiplication in its vertebrate hosts is restricted to erythrocytes. Current knowledge of invasion of these target cells is limited. An efficient in vitro invasion assay was set up to gain access to this information. Parasites prepared from infected RBC, lysed by electroporation, and mixed with bovine RBC in a sele...

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Babesia divergens–like Infection, Washington State

Most reported U.S. zoonotic cases of babesiosis have occurred in the Northeast and been caused by Babesia microti. In Washington State, three cases of babesiosis have been reported previously, which were caused by WA1 (for "Washington 1")-type parasites. We investigated a case of babesiosis in Washington in an 82-year-old man whose spleen had been removed and whose parasitemia level was 41.4%. ...

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ژورنال

عنوان ژورنال: Veterinary Research

سال: 2004

ISSN: 0928-4249,1297-9716

DOI: 10.1051/vetres:2003047